Bi525 features a narrow passband combined with high peak transmission and excellent out-of-band blocking. It succeeds and improves upon both Bi518 and Bi520 bandpass filters, particularly when it comes to blocking in the near-infrared spectrum. Bi525 filters are typically used in applications employing 520nm green laser diodes or 520nm/525nm green LED light sources. The latter typically finds use in machine vision inspection while the former is commonly used in bioanalytical applications. Various popularly used fluorophores (e.g., GFP, GelStar, SYBER Green, etc.) have emission maximas at or very near 525nm, and Bi525 filters can be used to effectively block excitation sources as well as other interfering longer and shorter wavelength light while letting desired green emission wavelengths pass.